I'm curious to know about the E85 question as well. It doesn't take a full tank of E85 to reap the benefits of the increased octane rating. With as little of a 25% E85 content in a regular fillup provides an imense benefit in tunability and knock count reduction. I'm not sure if any tuners have played with that option (partial tank of E85), but I'm not sure of the benefits on an NA motor. My previous experience was with a Mazdaspeed 3, and the amount of timing you could add because of the "E-blend" was pretty amazing.
So if anyone with knowledge of tuning these motors, or any NA motor for that matter, is an increase of a few degrees of timing a big power increase for us?
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